Angelique Kerber was the biggest casualty at the WTA Western and Southern Open on Wednesday as defending champion Karolina Pliskova reached the round of 16.
German third seed and former world number one Kerber was bundled out after Ekaterina Makarova converted her eighth match point in Cincinnati.
World number one Pliskova booked her spot in the last 16 with a routine victory over Natalia Vikhlyantseva.
Grand slam winner Petra Kvitova suffered another defeat to Sloane Stephens as the 14th seed departed the WTA Premier tournament, while American veteran Venus Williams also said goodbye.
KERBER UPSET IN CINCINNATI
It was a marathon but Kerber could not go distance, beaten 6-4 1-6 7-6 (13-11).
Makarova needed an eighth match point to close out the contest against the 2016 runner-up.
The win was Makarova's second of the year over Kerber – who did not look happy post-match after the Russian called the trainer multiple times throughout – having triumphed at the French Open.
STEPHENS TOPPLES KVITOVA
Makarova will go head-to-head with Stephens in the last 16 after the American dispatched two-time Wimbledon champion 6-2 6-3.
For the second time in as many weeks, Stephens got the better of Kvitova, having beaten the Czech in Toronto en route to the semi-finals.
Stephens needed just 70 minutes to progress.
PLISKOVA OFF TO WINNING START
Back to defend her crown, Pliskova was a comprehensive 6-2 6-3 winner.
Pliskova's success in Cincinnati a year ago inspired her run to the 2016 US Open final, while she was a quarter-finalist at last week's Rogers Cup.
She broke in each set to secure a clash with Italian Camila Giorgi, who overcame Dario Gavrilova 7-6 (7-1) 5-7 6-3.
VENUS STUNNED BY BARTY
Enjoying a breakthrough year in 2017, claiming her first WTA Tour title, Barty upstaged Williams 6-3 2-6 6-2.
Australian qualifier Barty had previously lost all six matches against top-10 opponents but that changed thanks to her victory over the ninth seed.
Caroline Wozniacki, Madison Keys and Elina Svitolina were among those to win through.
|Madrid can overturn deficit as captain Ramos remains calm|
|Neymar wouldn´t have treated Messi like he did Cavani – Forlan|
|Twins´ Dozier calls for mandatory protective netting after young fan hit by foul ball|
|Bengals boss Lewis warns players about their job security|
|76ers set no limits on Simmons|
|Zidane not worried after Madrid lose ground on Barca|
|Casemiro calls for Madrid calm after shock Betis loss|
|Sane sends message to Guardiola as Manchester City shake off West Brom|
|Keep calm and carry on – Allegri backs Higuain to rediscover scoring touch|
|Bosz proud of table-topping Dortmund after Hamburg triumph|
|Marlins slugger Stanton crushes 56th home run|
|Montella points to schedule after uninspired Milan win|
|Conte confirms Luiz will be fit to face Atletico|
|Manchester City boss Guardiola hopeful Gundogan injury not serious|
|Real Madrid 0 Real Betis 1: Ronaldo´s return ruined as Sanabria stuns LaLiga champions|
|Wilshere is ready for the league - Wenger|
|Simeone lauds ´different class´ Griezmann|
|Mourinho praises United´s professionalism in EFL Cup win|
|Spurs to host West Ham in EFL Cup fourth round|
|Froome: Time to put my feet up|
|West Brom 1 Manchester City 2: Sane at the double amid fresh Gundogan fears|
|Manchester United 4 Burton Albion 1: Mourinho´s men begin EFL Cup defence in style|
|Arsenal 1 Doncaster Rovers 0: Walcott hits winner for Wenger´s men|
|AC Milan 2 SPAL 0: Penalty double seals comfortable win for Montella´s side|
|Chelsea 5 Nottingham Forest 1: Hazard on form as Batshuayi claims hat-trick|
|Juventus 1 Fiorentina 0: Mandzukic on target as Bianconeri maintain winning start|
|Returning Gundogan limps out of impressive Manchester City return|
|Hamburg 0 Borussia Dortmund 3: Bosz´s men cruise back to Bundesliga summit|
|Broady stuns Mannarino in St Petersburg|
|Athletic Bilbao 1 Atletico Madrid 2: Correa and Carrasco strike in second-half surge|
|Simon suffers surprise Moselle Open exit as home hopes struggle in Metz|
|South Africa announce India schedule and planned four-day Zimbabwe Test|
|I have nothing to prove - Paulinho|
|Prodl pens new four-year Watford contract|
|Monaco renew Subasic, Raggi deals|
|Stoneman is ready for Ashes test - Cook|
|Sampson sacked as England Women´s boss|
|Manchester United to act against ´offensive behaviour´ after Lukaku race row|
|Atletico Madrid´s Wanda Metropolitano to host 2019 Champions League final|
|Flying Dutchman Dumoulin crowned world champion|
|Wickmayer topples Peng in Guangzhou, Hogenkamp makes Seoul progress|
|Solomona focused on Sale rather than England return|
|‘Raging Bull’ Jake LaMotta dies aged 95|
|Chelsea confident of recruitment compliance amid FIFA investigation|
|Brighton seal permanent signing of Newcastle keeper Krul|
|Louw, Nkosi and Schreuder get Springboks call|
|Perez donates 3m pesos to Mexico earthquake fund|
|Injured Mathews out of opening Pakistan Test|
|Double leg break victim Rossi to attempt incredible Aragon GP return|
|Barcelona condemn Spanish crackdown on Catalonia independence vote|
|Muguruza marches past Puig, Pliskova and Kerber progress in Tokyo|
|Benzema seals Real Madrid stay until 2021|
|Smith defends captaincy ahead of landmark ODI|
|Perisic promised Inter can match Manchester United|
|The race is on - Aubameyang lays down Lewandowski challenge|
|Cristiano Ronaldo´s Real Madrid return confirmed|
|Bellew tells Haye ´I oblige you´|
|Mbappe can prove he´s ready for Madrid at PSG - Morientes|
|Five-year ban for Pakistan batsman Latif|
|PSG ´destroyed the market´ with Neymar and Mbappe - Elber|
|Bulldogs chairman says sacked Hasler will not be compensated|
|Coutinho withdrawal inspired Leicester to victory, claims Shakespeare|
|Windies opener Brathwaite cleared to bowl|
|Valverde hopes Suarez benefits from Eibar rest|
|Wilshere returning to his best form - Wenger|
|Yankees, Cardinals keep pace in postseason race|
|Klopp: Liverpool are still a good football team|
|Pochettino hails ´top player´ Alli|
|Messi from ´another planet´, says Semedo|
|James performance pleases Ancelotti|